vitronectin coated 48 well plate Search Results


90
Corning Life Sciences vitronectin coated flasks
Vitronectin Coated Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitronectin+coated+48+well+plate/vitronectin+coated+12+well+plates/pmc08284774-56-25-26
Average 90 stars, based on 1 article reviews
vitronectin coated flasks - by Bioz Stars, 2026-09
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Cytomatrix Pty Ltd vitronectin-coated 96-well plates
Vitronectin Coated 96 Well Plates, supplied by Cytomatrix Pty Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitronectin+coated+48+well+plate/vitronectin+coated+96+well+plates/pmc06495722-111-7-10
Average 90 stars, based on 1 article reviews
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Promega 96-well plates coated with 500 ng human vitronectin
96 Well Plates Coated With 500 Ng Human Vitronectin, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitronectin+coated+48+well+plate/96+well+plates+coated+with+500+ng+human+vitronectin/pm11437656-196-15-17
Average 90 stars, based on 1 article reviews
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Corning Life Sciences 24-well tissue culture plates uncoated/coated with vitronectin (vn)
DU145 cells adhesion, invasion and intracellular activated pathways upon exposure to LO145 or LOR80. a DU145 adhesion, expressed as % of adherent cells, upon exposure to LO145 or LOR80 vs vehicle (considered as 100% control) and evaluated both in absence or presence of <t>vitronectin</t> (VN) for either 30 or 60 min. Stat. significances are expressed on vehicle. b DU145 adhesion measured as above, on VN after 60′ of exposure to LO145 or LOR80, untreated or pre-treated for 45′ RT with anti-αV-integrin monoclonal blocking antibody (anti-αV Ab) and indicated as LO145 αv and LOR80 αv , respectively; as negative control cells were treated by the anti-αV Ab alone. (LO145 αv / LO145)**; (LOR80 αv / LOR80)***; (LOR80/ vehicle) ***. c Western blotting analysis of pFAK, FAK, pAKT, AKT and γ-tubulin on cell lysates from DU145 untreated (CTR), or treated with LO145 or LOR80, alone or pre-exposed to anti-αV Ab (see above). pFAK and pAKT quantification is also reported. d Adhesion of DU145 measured as above, untreated (considered as 100%) or pretreated with the AKT inhibitor GDC-0068 for 16 h, were exposed to vehicle, LO145 or LOR80. (GDC-0068/ vehicle)***; (LOR80/ vehicle) **; (GDC-0068+ LOR80/ LOR80)* ( e ) DU145 invasion, expressed as % of invading cells, upon 16 h exposure to LOR80 treated with anti-αV Ab (LOR80 av ) or control Ab (LOR80 CTR Ab ) vs vehicle (considered as 100% control); as negative control cells were treated by CTR Ab alone (anti-pAKT antibody ineffective on non-permeabilized intact cells); (LOR80/ vehicle)***; (LOR80 CTR Ab / vehicle)***; (LOR80 av / LOR80)** ( f ) Invasion of DU145 measured as above, untreated (considered as 100%), or pretreated with the AKT inhibitor GDC-0068 for 6 h and exposed to vehicle, LO145 or LOR80;(GDC-0068/ vehicle)***; (LOR80/ vehicle)***; (GDC-0068 + LOR80/ LOR80)***. Results are representative of a single experiment and several experiments yielded similar results ( b ), or are the average of at least two experiments ( a ; d ; e ; f )
24 Well Tissue Culture Plates Uncoated/Coated With Vitronectin (Vn), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitronectin+coated+48+well+plate/24+well+tissue+culture+plates+uncoated+coated+with+vitronectin++vn+/pmc06639931-75-12-14
Average 90 stars, based on 1 article reviews
24-well tissue culture plates uncoated/coated with vitronectin (vn) - by Bioz Stars, 2026-09
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STEMCELL Technologies Inc 6-well culture plate coated with vitronectin xftm
DU145 cells adhesion, invasion and intracellular activated pathways upon exposure to LO145 or LOR80. a DU145 adhesion, expressed as % of adherent cells, upon exposure to LO145 or LOR80 vs vehicle (considered as 100% control) and evaluated both in absence or presence of <t>vitronectin</t> (VN) for either 30 or 60 min. Stat. significances are expressed on vehicle. b DU145 adhesion measured as above, on VN after 60′ of exposure to LO145 or LOR80, untreated or pre-treated for 45′ RT with anti-αV-integrin monoclonal blocking antibody (anti-αV Ab) and indicated as LO145 αv and LOR80 αv , respectively; as negative control cells were treated by the anti-αV Ab alone. (LO145 αv / LO145)**; (LOR80 αv / LOR80)***; (LOR80/ vehicle) ***. c Western blotting analysis of pFAK, FAK, pAKT, AKT and γ-tubulin on cell lysates from DU145 untreated (CTR), or treated with LO145 or LOR80, alone or pre-exposed to anti-αV Ab (see above). pFAK and pAKT quantification is also reported. d Adhesion of DU145 measured as above, untreated (considered as 100%) or pretreated with the AKT inhibitor GDC-0068 for 16 h, were exposed to vehicle, LO145 or LOR80. (GDC-0068/ vehicle)***; (LOR80/ vehicle) **; (GDC-0068+ LOR80/ LOR80)* ( e ) DU145 invasion, expressed as % of invading cells, upon 16 h exposure to LOR80 treated with anti-αV Ab (LOR80 av ) or control Ab (LOR80 CTR Ab ) vs vehicle (considered as 100% control); as negative control cells were treated by CTR Ab alone (anti-pAKT antibody ineffective on non-permeabilized intact cells); (LOR80/ vehicle)***; (LOR80 CTR Ab / vehicle)***; (LOR80 av / LOR80)** ( f ) Invasion of DU145 measured as above, untreated (considered as 100%), or pretreated with the AKT inhibitor GDC-0068 for 6 h and exposed to vehicle, LO145 or LOR80;(GDC-0068/ vehicle)***; (LOR80/ vehicle)***; (GDC-0068 + LOR80/ LOR80)***. Results are representative of a single experiment and several experiments yielded similar results ( b ), or are the average of at least two experiments ( a ; d ; e ; f )
6 Well Culture Plate Coated With Vitronectin Xftm, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitronectin+coated+48+well+plate/6+well+culture+plate+coated+with+vitronectin+xftm/pmc08129508-50-13-15
Average 90 stars, based on 1 article reviews
6-well culture plate coated with vitronectin xftm - by Bioz Stars, 2026-09
90/100 stars
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90
STEMCELL Technologies Inc 6-well plates coated with vitronectin in celladhere buffer
DU145 cells adhesion, invasion and intracellular activated pathways upon exposure to LO145 or LOR80. a DU145 adhesion, expressed as % of adherent cells, upon exposure to LO145 or LOR80 vs vehicle (considered as 100% control) and evaluated both in absence or presence of <t>vitronectin</t> (VN) for either 30 or 60 min. Stat. significances are expressed on vehicle. b DU145 adhesion measured as above, on VN after 60′ of exposure to LO145 or LOR80, untreated or pre-treated for 45′ RT with anti-αV-integrin monoclonal blocking antibody (anti-αV Ab) and indicated as LO145 αv and LOR80 αv , respectively; as negative control cells were treated by the anti-αV Ab alone. (LO145 αv / LO145)**; (LOR80 αv / LOR80)***; (LOR80/ vehicle) ***. c Western blotting analysis of pFAK, FAK, pAKT, AKT and γ-tubulin on cell lysates from DU145 untreated (CTR), or treated with LO145 or LOR80, alone or pre-exposed to anti-αV Ab (see above). pFAK and pAKT quantification is also reported. d Adhesion of DU145 measured as above, untreated (considered as 100%) or pretreated with the AKT inhibitor GDC-0068 for 16 h, were exposed to vehicle, LO145 or LOR80. (GDC-0068/ vehicle)***; (LOR80/ vehicle) **; (GDC-0068+ LOR80/ LOR80)* ( e ) DU145 invasion, expressed as % of invading cells, upon 16 h exposure to LOR80 treated with anti-αV Ab (LOR80 av ) or control Ab (LOR80 CTR Ab ) vs vehicle (considered as 100% control); as negative control cells were treated by CTR Ab alone (anti-pAKT antibody ineffective on non-permeabilized intact cells); (LOR80/ vehicle)***; (LOR80 CTR Ab / vehicle)***; (LOR80 av / LOR80)** ( f ) Invasion of DU145 measured as above, untreated (considered as 100%), or pretreated with the AKT inhibitor GDC-0068 for 6 h and exposed to vehicle, LO145 or LOR80;(GDC-0068/ vehicle)***; (LOR80/ vehicle)***; (GDC-0068 + LOR80/ LOR80)***. Results are representative of a single experiment and several experiments yielded similar results ( b ), or are the average of at least two experiments ( a ; d ; e ; f )
6 Well Plates Coated With Vitronectin In Celladhere Buffer, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitronectin+coated+48+well+plate/6+well+plates+coated+with+vitronectin+in+celladhere+buffer/pmc08531667-59-11-12
Average 90 stars, based on 1 article reviews
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90/100 stars
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90
STEMCELL Technologies Inc 24-well plates coated with vitronectin 07180
DU145 cells adhesion, invasion and intracellular activated pathways upon exposure to LO145 or LOR80. a DU145 adhesion, expressed as % of adherent cells, upon exposure to LO145 or LOR80 vs vehicle (considered as 100% control) and evaluated both in absence or presence of <t>vitronectin</t> (VN) for either 30 or 60 min. Stat. significances are expressed on vehicle. b DU145 adhesion measured as above, on VN after 60′ of exposure to LO145 or LOR80, untreated or pre-treated for 45′ RT with anti-αV-integrin monoclonal blocking antibody (anti-αV Ab) and indicated as LO145 αv and LOR80 αv , respectively; as negative control cells were treated by the anti-αV Ab alone. (LO145 αv / LO145)**; (LOR80 αv / LOR80)***; (LOR80/ vehicle) ***. c Western blotting analysis of pFAK, FAK, pAKT, AKT and γ-tubulin on cell lysates from DU145 untreated (CTR), or treated with LO145 or LOR80, alone or pre-exposed to anti-αV Ab (see above). pFAK and pAKT quantification is also reported. d Adhesion of DU145 measured as above, untreated (considered as 100%) or pretreated with the AKT inhibitor GDC-0068 for 16 h, were exposed to vehicle, LO145 or LOR80. (GDC-0068/ vehicle)***; (LOR80/ vehicle) **; (GDC-0068+ LOR80/ LOR80)* ( e ) DU145 invasion, expressed as % of invading cells, upon 16 h exposure to LOR80 treated with anti-αV Ab (LOR80 av ) or control Ab (LOR80 CTR Ab ) vs vehicle (considered as 100% control); as negative control cells were treated by CTR Ab alone (anti-pAKT antibody ineffective on non-permeabilized intact cells); (LOR80/ vehicle)***; (LOR80 CTR Ab / vehicle)***; (LOR80 av / LOR80)** ( f ) Invasion of DU145 measured as above, untreated (considered as 100%), or pretreated with the AKT inhibitor GDC-0068 for 6 h and exposed to vehicle, LO145 or LOR80;(GDC-0068/ vehicle)***; (LOR80/ vehicle)***; (GDC-0068 + LOR80/ LOR80)***. Results are representative of a single experiment and several experiments yielded similar results ( b ), or are the average of at least two experiments ( a ; d ; e ; f )
24 Well Plates Coated With Vitronectin 07180, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitronectin+coated+48+well+plate/24+well+plates+coated+with+vitronectin+07180/pmc09379358-129-5-7
Average 90 stars, based on 1 article reviews
24-well plates coated with vitronectin 07180 - by Bioz Stars, 2026-09
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90
Becton Dickinson vitronectin-coated 6-well plates
DU145 cells adhesion, invasion and intracellular activated pathways upon exposure to LO145 or LOR80. a DU145 adhesion, expressed as % of adherent cells, upon exposure to LO145 or LOR80 vs vehicle (considered as 100% control) and evaluated both in absence or presence of <t>vitronectin</t> (VN) for either 30 or 60 min. Stat. significances are expressed on vehicle. b DU145 adhesion measured as above, on VN after 60′ of exposure to LO145 or LOR80, untreated or pre-treated for 45′ RT with anti-αV-integrin monoclonal blocking antibody (anti-αV Ab) and indicated as LO145 αv and LOR80 αv , respectively; as negative control cells were treated by the anti-αV Ab alone. (LO145 αv / LO145)**; (LOR80 αv / LOR80)***; (LOR80/ vehicle) ***. c Western blotting analysis of pFAK, FAK, pAKT, AKT and γ-tubulin on cell lysates from DU145 untreated (CTR), or treated with LO145 or LOR80, alone or pre-exposed to anti-αV Ab (see above). pFAK and pAKT quantification is also reported. d Adhesion of DU145 measured as above, untreated (considered as 100%) or pretreated with the AKT inhibitor GDC-0068 for 16 h, were exposed to vehicle, LO145 or LOR80. (GDC-0068/ vehicle)***; (LOR80/ vehicle) **; (GDC-0068+ LOR80/ LOR80)* ( e ) DU145 invasion, expressed as % of invading cells, upon 16 h exposure to LOR80 treated with anti-αV Ab (LOR80 av ) or control Ab (LOR80 CTR Ab ) vs vehicle (considered as 100% control); as negative control cells were treated by CTR Ab alone (anti-pAKT antibody ineffective on non-permeabilized intact cells); (LOR80/ vehicle)***; (LOR80 CTR Ab / vehicle)***; (LOR80 av / LOR80)** ( f ) Invasion of DU145 measured as above, untreated (considered as 100%), or pretreated with the AKT inhibitor GDC-0068 for 6 h and exposed to vehicle, LO145 or LOR80;(GDC-0068/ vehicle)***; (LOR80/ vehicle)***; (GDC-0068 + LOR80/ LOR80)***. Results are representative of a single experiment and several experiments yielded similar results ( b ), or are the average of at least two experiments ( a ; d ; e ; f )
Vitronectin Coated 6 Well Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitronectin+coated+48+well+plate/vitronectin+coated+6+well+plates/pm36521659-170-15-17
Average 90 stars, based on 1 article reviews
vitronectin-coated 6-well plates - by Bioz Stars, 2026-09
90/100 stars
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90
Promega 96-well elisa plates coated with vitronectin
DU145 cells adhesion, invasion and intracellular activated pathways upon exposure to LO145 or LOR80. a DU145 adhesion, expressed as % of adherent cells, upon exposure to LO145 or LOR80 vs vehicle (considered as 100% control) and evaluated both in absence or presence of <t>vitronectin</t> (VN) for either 30 or 60 min. Stat. significances are expressed on vehicle. b DU145 adhesion measured as above, on VN after 60′ of exposure to LO145 or LOR80, untreated or pre-treated for 45′ RT with anti-αV-integrin monoclonal blocking antibody (anti-αV Ab) and indicated as LO145 αv and LOR80 αv , respectively; as negative control cells were treated by the anti-αV Ab alone. (LO145 αv / LO145)**; (LOR80 αv / LOR80)***; (LOR80/ vehicle) ***. c Western blotting analysis of pFAK, FAK, pAKT, AKT and γ-tubulin on cell lysates from DU145 untreated (CTR), or treated with LO145 or LOR80, alone or pre-exposed to anti-αV Ab (see above). pFAK and pAKT quantification is also reported. d Adhesion of DU145 measured as above, untreated (considered as 100%) or pretreated with the AKT inhibitor GDC-0068 for 16 h, were exposed to vehicle, LO145 or LOR80. (GDC-0068/ vehicle)***; (LOR80/ vehicle) **; (GDC-0068+ LOR80/ LOR80)* ( e ) DU145 invasion, expressed as % of invading cells, upon 16 h exposure to LOR80 treated with anti-αV Ab (LOR80 av ) or control Ab (LOR80 CTR Ab ) vs vehicle (considered as 100% control); as negative control cells were treated by CTR Ab alone (anti-pAKT antibody ineffective on non-permeabilized intact cells); (LOR80/ vehicle)***; (LOR80 CTR Ab / vehicle)***; (LOR80 av / LOR80)** ( f ) Invasion of DU145 measured as above, untreated (considered as 100%), or pretreated with the AKT inhibitor GDC-0068 for 6 h and exposed to vehicle, LO145 or LOR80;(GDC-0068/ vehicle)***; (LOR80/ vehicle)***; (GDC-0068 + LOR80/ LOR80)***. Results are representative of a single experiment and several experiments yielded similar results ( b ), or are the average of at least two experiments ( a ; d ; e ; f )
96 Well Elisa Plates Coated With Vitronectin, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitronectin+coated+48+well+plate/96+well+elisa+plates+coated+with+vitronectin/10__1161_slash_circresaha__108__178434-219-14-32
Average 90 stars, based on 1 article reviews
96-well elisa plates coated with vitronectin - by Bioz Stars, 2026-09
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90
STEMCELL Technologies Inc vitronectin-coated 96-well plate
DU145 cells adhesion, invasion and intracellular activated pathways upon exposure to LO145 or LOR80. a DU145 adhesion, expressed as % of adherent cells, upon exposure to LO145 or LOR80 vs vehicle (considered as 100% control) and evaluated both in absence or presence of <t>vitronectin</t> (VN) for either 30 or 60 min. Stat. significances are expressed on vehicle. b DU145 adhesion measured as above, on VN after 60′ of exposure to LO145 or LOR80, untreated or pre-treated for 45′ RT with anti-αV-integrin monoclonal blocking antibody (anti-αV Ab) and indicated as LO145 αv and LOR80 αv , respectively; as negative control cells were treated by the anti-αV Ab alone. (LO145 αv / LO145)**; (LOR80 αv / LOR80)***; (LOR80/ vehicle) ***. c Western blotting analysis of pFAK, FAK, pAKT, AKT and γ-tubulin on cell lysates from DU145 untreated (CTR), or treated with LO145 or LOR80, alone or pre-exposed to anti-αV Ab (see above). pFAK and pAKT quantification is also reported. d Adhesion of DU145 measured as above, untreated (considered as 100%) or pretreated with the AKT inhibitor GDC-0068 for 16 h, were exposed to vehicle, LO145 or LOR80. (GDC-0068/ vehicle)***; (LOR80/ vehicle) **; (GDC-0068+ LOR80/ LOR80)* ( e ) DU145 invasion, expressed as % of invading cells, upon 16 h exposure to LOR80 treated with anti-αV Ab (LOR80 av ) or control Ab (LOR80 CTR Ab ) vs vehicle (considered as 100% control); as negative control cells were treated by CTR Ab alone (anti-pAKT antibody ineffective on non-permeabilized intact cells); (LOR80/ vehicle)***; (LOR80 CTR Ab / vehicle)***; (LOR80 av / LOR80)** ( f ) Invasion of DU145 measured as above, untreated (considered as 100%), or pretreated with the AKT inhibitor GDC-0068 for 6 h and exposed to vehicle, LO145 or LOR80;(GDC-0068/ vehicle)***; (LOR80/ vehicle)***; (GDC-0068 + LOR80/ LOR80)***. Results are representative of a single experiment and several experiments yielded similar results ( b ), or are the average of at least two experiments ( a ; d ; e ; f )
Vitronectin Coated 96 Well Plate, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitronectin+coated+48+well+plate/vitronectin+coated+96+well+plate/pm35472001-319-13-20
Average 90 stars, based on 1 article reviews
vitronectin-coated 96-well plate - by Bioz Stars, 2026-09
90/100 stars
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90
Becton Dickinson vitronectin-coated 24-well plates
DU145 cells adhesion, invasion and intracellular activated pathways upon exposure to LO145 or LOR80. a DU145 adhesion, expressed as % of adherent cells, upon exposure to LO145 or LOR80 vs vehicle (considered as 100% control) and evaluated both in absence or presence of <t>vitronectin</t> (VN) for either 30 or 60 min. Stat. significances are expressed on vehicle. b DU145 adhesion measured as above, on VN after 60′ of exposure to LO145 or LOR80, untreated or pre-treated for 45′ RT with anti-αV-integrin monoclonal blocking antibody (anti-αV Ab) and indicated as LO145 αv and LOR80 αv , respectively; as negative control cells were treated by the anti-αV Ab alone. (LO145 αv / LO145)**; (LOR80 αv / LOR80)***; (LOR80/ vehicle) ***. c Western blotting analysis of pFAK, FAK, pAKT, AKT and γ-tubulin on cell lysates from DU145 untreated (CTR), or treated with LO145 or LOR80, alone or pre-exposed to anti-αV Ab (see above). pFAK and pAKT quantification is also reported. d Adhesion of DU145 measured as above, untreated (considered as 100%) or pretreated with the AKT inhibitor GDC-0068 for 16 h, were exposed to vehicle, LO145 or LOR80. (GDC-0068/ vehicle)***; (LOR80/ vehicle) **; (GDC-0068+ LOR80/ LOR80)* ( e ) DU145 invasion, expressed as % of invading cells, upon 16 h exposure to LOR80 treated with anti-αV Ab (LOR80 av ) or control Ab (LOR80 CTR Ab ) vs vehicle (considered as 100% control); as negative control cells were treated by CTR Ab alone (anti-pAKT antibody ineffective on non-permeabilized intact cells); (LOR80/ vehicle)***; (LOR80 CTR Ab / vehicle)***; (LOR80 av / LOR80)** ( f ) Invasion of DU145 measured as above, untreated (considered as 100%), or pretreated with the AKT inhibitor GDC-0068 for 6 h and exposed to vehicle, LO145 or LOR80;(GDC-0068/ vehicle)***; (LOR80/ vehicle)***; (GDC-0068 + LOR80/ LOR80)***. Results are representative of a single experiment and several experiments yielded similar results ( b ), or are the average of at least two experiments ( a ; d ; e ; f )
Vitronectin Coated 24 Well Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitronectin+coated+48+well+plate/vitronectin+coated+24+well+plates/pm23207252-67-4-11
Average 90 stars, based on 1 article reviews
vitronectin-coated 24-well plates - by Bioz Stars, 2026-09
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DU145 cells adhesion, invasion and intracellular activated pathways upon exposure to LO145 or LOR80. a DU145 adhesion, expressed as % of adherent cells, upon exposure to LO145 or LOR80 vs vehicle (considered as 100% control) and evaluated both in absence or presence of vitronectin (VN) for either 30 or 60 min. Stat. significances are expressed on vehicle. b DU145 adhesion measured as above, on VN after 60′ of exposure to LO145 or LOR80, untreated or pre-treated for 45′ RT with anti-αV-integrin monoclonal blocking antibody (anti-αV Ab) and indicated as LO145 αv and LOR80 αv , respectively; as negative control cells were treated by the anti-αV Ab alone. (LO145 αv / LO145)**; (LOR80 αv / LOR80)***; (LOR80/ vehicle) ***. c Western blotting analysis of pFAK, FAK, pAKT, AKT and γ-tubulin on cell lysates from DU145 untreated (CTR), or treated with LO145 or LOR80, alone or pre-exposed to anti-αV Ab (see above). pFAK and pAKT quantification is also reported. d Adhesion of DU145 measured as above, untreated (considered as 100%) or pretreated with the AKT inhibitor GDC-0068 for 16 h, were exposed to vehicle, LO145 or LOR80. (GDC-0068/ vehicle)***; (LOR80/ vehicle) **; (GDC-0068+ LOR80/ LOR80)* ( e ) DU145 invasion, expressed as % of invading cells, upon 16 h exposure to LOR80 treated with anti-αV Ab (LOR80 av ) or control Ab (LOR80 CTR Ab ) vs vehicle (considered as 100% control); as negative control cells were treated by CTR Ab alone (anti-pAKT antibody ineffective on non-permeabilized intact cells); (LOR80/ vehicle)***; (LOR80 CTR Ab / vehicle)***; (LOR80 av / LOR80)** ( f ) Invasion of DU145 measured as above, untreated (considered as 100%), or pretreated with the AKT inhibitor GDC-0068 for 6 h and exposed to vehicle, LO145 or LOR80;(GDC-0068/ vehicle)***; (LOR80/ vehicle)***; (GDC-0068 + LOR80/ LOR80)***. Results are representative of a single experiment and several experiments yielded similar results ( b ), or are the average of at least two experiments ( a ; d ; e ; f )

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Large oncosomes overexpressing integrin alpha-V promote prostate cancer adhesion and invasion via AKT activation

doi: 10.1186/s13046-019-1317-6

Figure Lengend Snippet: DU145 cells adhesion, invasion and intracellular activated pathways upon exposure to LO145 or LOR80. a DU145 adhesion, expressed as % of adherent cells, upon exposure to LO145 or LOR80 vs vehicle (considered as 100% control) and evaluated both in absence or presence of vitronectin (VN) for either 30 or 60 min. Stat. significances are expressed on vehicle. b DU145 adhesion measured as above, on VN after 60′ of exposure to LO145 or LOR80, untreated or pre-treated for 45′ RT with anti-αV-integrin monoclonal blocking antibody (anti-αV Ab) and indicated as LO145 αv and LOR80 αv , respectively; as negative control cells were treated by the anti-αV Ab alone. (LO145 αv / LO145)**; (LOR80 αv / LOR80)***; (LOR80/ vehicle) ***. c Western blotting analysis of pFAK, FAK, pAKT, AKT and γ-tubulin on cell lysates from DU145 untreated (CTR), or treated with LO145 or LOR80, alone or pre-exposed to anti-αV Ab (see above). pFAK and pAKT quantification is also reported. d Adhesion of DU145 measured as above, untreated (considered as 100%) or pretreated with the AKT inhibitor GDC-0068 for 16 h, were exposed to vehicle, LO145 or LOR80. (GDC-0068/ vehicle)***; (LOR80/ vehicle) **; (GDC-0068+ LOR80/ LOR80)* ( e ) DU145 invasion, expressed as % of invading cells, upon 16 h exposure to LOR80 treated with anti-αV Ab (LOR80 av ) or control Ab (LOR80 CTR Ab ) vs vehicle (considered as 100% control); as negative control cells were treated by CTR Ab alone (anti-pAKT antibody ineffective on non-permeabilized intact cells); (LOR80/ vehicle)***; (LOR80 CTR Ab / vehicle)***; (LOR80 av / LOR80)** ( f ) Invasion of DU145 measured as above, untreated (considered as 100%), or pretreated with the AKT inhibitor GDC-0068 for 6 h and exposed to vehicle, LO145 or LOR80;(GDC-0068/ vehicle)***; (LOR80/ vehicle)***; (GDC-0068 + LOR80/ LOR80)***. Results are representative of a single experiment and several experiments yielded similar results ( b ), or are the average of at least two experiments ( a ; d ; e ; f )

Article Snippet: Cell adhesion assays were performed using 24-well tissue culture plates uncoated/coated with vitronectin (VN) (Corning, NY 14831 USA).

Techniques: Control, Blocking Assay, Negative Control, Western Blot